Chloral hydrate anesthesia in mice

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August undefined, 2024

WebHepatic enzyme analysis: Chloral hydrate did not significantly induce either lauric acid 4-hydroxylase activity or CYP4A immunoreactive protein in any of the dosed groups of ad libitum-fed mice. However, 100 mg/kg did significantly induce both lauric acid 4-hydroxylase activity and CYP4A immunoreactive protein in the dietary-controlled mice. WebMar 5, 2009 · For surgery and microinjection, chloral hydrate-anesthetized mice were positioned in a stereotaxic apparatus (NARISHIGE Scientific Instrument LAB); the lentivirus filled in the glass electrode was injected aiming at PAG (AP-4.5 mm; ML ±0 mm; DV 2mm). Lentivirus (1.5 μL) was microinjected into the PAG at a rate of 0.15 μL/min.

Chloral Hydrate Anesthesia and Lens Opacification in Mice

WebJul 1, 1993 · Some studies (55,56) use 300-400 mg/kg chloral hydrate to perform surgery or establish animal models, the process of which requires deep anesthesia and long duration. ... WebPurpose: To study the effect of chloral hydrate in the eye lens in mice.Methods: We instilled tropicamide in the eyes of two groups of eight 129 strain wild-type mice and … lockingtons

A review of laboratory animal anesthesia with chloral hydrate …

WebSep 30, 2013 · The mice used in this study were between 6 weeks and 4 months of age and were on a randomly mixed genetic background comprised of C57BL/6J and 129 Sv/Ev strains. We observed only mild age-dependent ABR. Minimal differences in ABR thresholds and Wave I latencies for 2,2,2-tribromoethanol versus 2,2,2-tribromoethanol–chloral … Webmoethanol or fentanyl-fluanisone-diazepam anesthesia. However, a small but significant degree of radioprotection was observed under chloral hydrate or pentobarbital anes- ... (iii) Chloral hydrate: 2,2,2-trichloro-1,1-ethanediol (chloral hydrate by B.D.H. Chemicals Ltd.). The mice were boxed 5 min after an intraperitoneal injection of 300 mg/kg ... WebApr 12, 2024 · The nude mice were weighed on day 15 of administration and anesthetized with 350 mg/kg chloral hydrate. Immediately after anesthesia, the nude mice were fixed on a foam board with a needle and perfused with 0.9% physiological saline to fill the perfusion tube, the infusion set was inserted into the left ventricle and the needle was … lockington road battersea

Mouse Anesthesia: The Art and Science - Oxford Academic

Chloral Hydrate Anesthesia and Lens Opacification in Mice

WebAll mice were intraperitoneally injected (10 mL·kg −1) with a 4% chloral hydrate solution for anesthesia. For ALI model group, the mice were instilled with 50 μL of LPS solution at a concentration of 1.5 mg·mL −1. (Dose was 3 mg·kg −1); the mice in the blank control group were instilled with the same volume of normal saline, and the ... WebWe show that chloral hydrate, when used intraperitoneally, induces acute and reversible lens opacification in all animals. In addition, we found that instillation of hypromellose … lockington shopsWebJun 12, 1998 · Chloral hydrate is a hepatocarcinogen in mice but not rats. To examine this species-related difference, male and female B6C3F1 mice and Fischer (F344) rats were treated by gavage with 1 or 12 doses of chloral hydrate, and concentrations of the drug and its metabolites were determined in plasma at 0.25, 7, 3, 6, and 24 h and 2, 4, 8, and 16 d … india vs indonesia thomas cup 2022

"WebMay 1, 2024 · 1302 Purpose: To evaluate the effect of different concentrations of chloral hydrate and isoflurane anesthesia on brain uptake of 18F-FDG in mice. Methods: Total … " - Chloral hydrate anesthesia in mice

Chloral hydrate anesthesia in mice

A review of tribromoethanol anesthesia for production of …

WebAll surgery was performed under chloral hydrate anesthesia, and all efforts were made to minimize suffering. ... All mice were sacrificed under chloral hydrate anesthesia at day 21 after the first injection. Tumor nodules in the abdominal cavity were collected and weighed; mice weight and ascites were also evaluated in all groups. WebDec 1, 2013 · To explore effect of different anesthesia methods and different anesthetics on erythrocyte immune function in mice. Methods. The mice were anesthetized by …

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WebFeb 17, 2009 · Anaesthesia was induced by chloral hydrate (500 mg kg −1, i.p.), and mice were killed 10 min after drug injection. Control mice received a PBS injection. Control mice received a PBS injection. Representative immunoblots are shown left to … WebJan 10, 2024 · Chloral hydrate . 400 mg/kg IP . Unproven . Non-survival procedures . Tribromoethanol . 125 - 250 mg/kg IP . 30 - 45 minutes . Avoid re-dosing ... Spaeni D, …

WebJun 1, 2009 · The mice of Groups A and D were injected by 4% chloral hydrate solution to anesthesia. The dose was 10 mL/kg [8, 19] . The mice in Groups B and E exposured to … WebJan 12, 2015 · The mice were housed and fed in an air-conditioned room on a 12-h light/dark cycle in a standard laboratory. ... the mice were injected intraperitoneally with 4.3% chloral hydrate (430 mg/kg, Pharmacy ... prior to intravitreal injection of mini-αA. After anesthesia, compound tropicamide eye drops (Shenyang Xingqi Pharmaceutical Co., …

WebSynthesised by Justin Liebig in 1832 chloral hydrate is one of the oldest synthetic agents. Since 1869 it has been in use for hypnotic or sedative purposes. Chloral hydrate was used a lot from the end of the 19th century to the middle of the 20th century. Since then chloral hydrate has been less fre … WebAug 1, 2009 · Mice were anesthetized by i.p. injection of chloral hydrate (300 mg/kg), a well-known anesthetic, which is widely used despite some concern on its use for anesthesia of small animals (28). It does ...

WebSep 30, 2013 · Rapid recovery of mice from 2,2,2-tribromoethanol–chloral hydrate anesthesia and normal weight maintenance. In addition to determining the effect of 2,2,2-tribromoethanol–chloral hydrate anesthesia on ABRs, we examined post-anesthesia recovery times and the safety of the anesthesia in terms of mouse weight over the …

WebMay 31, 2024 · It is worth noting that adverse side effects similar to those reported after TBE administration occur after i.p. administration of chloral hydrate and alpha-chloralose 23. … india vs indonesia thomas cup finalWebJul 19, 2024 · PDF The present experiments were performed to study the effects and time trends of different anesthetic agents on the left ventricular (LV) systolic... Find, read and cite all the research you ... lockington to melbourne airportWebAbstract. Purpose: The aim of the present study was to study the time-course development of cold cataract in mice under general anesthesia. Methods: We anesthetized five groups of 10 mice (12 weeks old) with 400 mg/Kg intraperitoneal injections of chloral hydrate and exposed them to 0, 7, 15, 23, and 37°C for 1 hr. Cataract development was ... lockington storage ottomanWebBehavioral/Systems/Cognitive GABAergicAfferentsActivateBothGABA A andGABA B ReceptorsinMouseSubstantiaNigraDopaminergicNeurons InVivo ElenaBrazhnik,FulvaShah ... india vs ireland 2nd t20 scorecardWebOct 1, 2014 · Naïve mice and animals with acute traumatic brain injury induced by controlled cortical impact (CCI) were randomized to the following euthanasia protocols (n=10-11/group): no anesthesia (NA), 1 min of 4 vol% isoflurane in room air (ISO), 3 min of a combination of 5 mg/kg midazolam, 0.05 mg/kg fentanyl, and 0.5 mg/kg medetomidine ... india vs ireland 2023WebJul 1, 1993 · Some studies (55,56) use 300-400 mg/kg chloral hydrate to perform surgery or establish animal models, the process of which requires deep anesthesia and long duration. ... lockingtons trailers immingham india vs ireland cricket live streaming